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For the past several decades, amphibian species worldwide have been in decline. A significant proportion of the decline seems to be due to the spread of the chytrid fungus, Batrachochytrium dendrobatidis. Chytrid sporangia reside within the epidermal cells of infected animals, animals that consequently show areas of sloughed skin. The infection cycle typically takes four to five days, at the end of which zoospores are released from sporangia into the environment.
When adult amphibian skin harbors populations of the bacterium Janthinobacterium lividum, chytrid infection seems to be inhibited. Which of the following is the best experimental design to test whether this inhibition is actually due to the presence of J. lividum?


Definitions:

Chapter 13

A form of bankruptcy in the United States that allows individuals with a regular income to develop a plan to repay all or part of their debts.

Repayment Plan

A strategy or arrangement agreed upon to pay back borrowed money, often detailing the time frame and amounts due.

Insolvent

The condition of being unable to pay debts as they fall due, or having liabilities that exceed the value of assets.

Relief

refers to legal remedies or aid provided through the court system to a person or entity that experiences harm or a legal wrong, aiming to restore rights or compensate losses.

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