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The Following Question Refers to the Figure

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The following question refers to the figure.
In this eight-year experiment, a population of E. coli, begun from a single cell, was grown in low-glucose conditions for 20,000 generations. Each culture was introduced to fresh growth medium every 24 hours. Occasionally, samples were removed from the population, and its ability to grow in low-glucose conditions was tested against that of members sampled from the ancestral (common ancestor) E. coli population. The following question refers to the figure. In this eight-year experiment, a population of E. coli, begun from a single cell, was grown in low-glucose conditions for 20,000 generations. Each culture was introduced to fresh growth medium every 24 hours. Occasionally, samples were removed from the population, and its ability to grow in low-glucose conditions was tested against that of members sampled from the ancestral (common ancestor)  E. coli population.   If the experimental population of E. coli lacks an F factor or F plasmid, and if bacteriophages are excluded from the bacterial cultures, how would beneficial mutations be transmitted horizontally to other E. coli cells? A)  sex pili B)  transduction C)  conjugation D)  transformation
If the experimental population of E. coli lacks an F factor or F plasmid, and if bacteriophages are excluded from the bacterial cultures, how would beneficial mutations be transmitted horizontally to other E. coli cells?


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