Which Order of the Following Steps Provides the Most Logical

question 37

Multiple Choice

Which order of the following steps provides the most logical sequence to follow when splicing foreign DNA into a plasmid and inserting the plasmid into a bacterium?
I. Transform bacteria with a recombinant DNA molecule.
II. Cut the plasmid DNA using restriction enzymes (endonucleases) .
III. Extract plasmid DNA from bacterial cells.
IV. Hydrogen-bond the plasmid DNA to non-plasmid DNA fragments.
V. Use ligase to seal plasmid DNA to non-plasmid DNA.

• A) II, III, V, IV, I
• B) III, II, IV, V, I
• C) III, IV, V, I, II
• D) IV, V, I, II, III

---

Definitions:

Specific Time Period

A definite or clearly defined stretch of time.

Theories

Theories are systematic frameworks composed of concepts and propositions intended to explain phenomena by specifying relations among variables, providing a coherent set of guidelines for understanding and predicting behavior or events.

Hypotheses

Proposed explanations for a phenomenon, set forth as a starting point for further investigation or experimentation.

Phenomenon

Phenomenon refers to an observable event, fact, or circumstance that can be scientifically investigated to gain understanding or evidence about it, often used to describe unusual or remarkable occurrences.