Which Order of the Following Steps Provides the Most Logical

question 37

Multiple Choice

Which order of the following steps provides the most logical sequence to follow when splicing foreign DNA into a plasmid and inserting the plasmid into a bacterium?
I. Transform bacteria with a recombinant DNA molecule.
II. Cut the plasmid DNA using restriction enzymes (endonucleases) .
III. Extract plasmid DNA from bacterial cells.
IV. Hydrogen-bond the plasmid DNA to non-plasmid DNA fragments.
V. Use ligase to seal plasmid DNA to non-plasmid DNA.

• A) II, III, V, IV, I
• B) III, II, IV, V, I
• C) III, IV, V, I, II
• D) IV, V, I, II, III

---

Definitions:

Mutual Assent

An agreement between parties that is reached when all involved parties have a matching understanding and intention to be bound by the terms.

Agreement Standard

The established criteria or clauses that form the basis of a contractual agreement between parties.

Acceptance

In contract law, it is the act of agreeing to the terms of an offer, thus creating a binding contract.

Commercial Reasonableness

A legal standard that measures the fairness of business practices, actions, or decisions in a commercial context.