Which of the Following Describes the Best Approach to Making

question 21

Multiple Choice

Which of the following describes the best approach to making a gene that contains introns shorter, but remaining functional, for genetic engineering purposes?

• A) express in bacteria then a restriction enzyme (endonuclease) to cut the gene into shorter pieces
• B) isolate the specific mRNA from the animal then use reverse transcriptase to produce cDNA
• C) isolate the specific protein from the animal then use the genetic code to reconstruct DNA from the polypeptide sequence
• D) using DNA ligase to put together fragments of the DNA that code for a particular polypeptide