A Researcher Inserted DNA Fragments from an Organism into Expression

question 58

Multiple Choice

A researcher inserted DNA fragments from an organism into expression vector plasmids and introduced the modified plasmids into bacterial cells. Which of the following methods would be an effective means of identifying which clones contain a specific gene of interest?

• A) Sequence the DNA of the plasmids from each isolate.
• B) Prepare and analyze restriction fragments of the plasmids.
• C) Isolate clones that still have the vector's genetic marker.
• D) Assay for activity of the gene product.
• E) Use reverse transcriptase to prepare cDNA copies.

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Definitions:

Cushing's Disease

A condition caused by an excess of cortisol in the body, often due to a tumor in the pituitary gland, leading to symptoms like weight gain, thinning skin, and hypertension.

Fat Deposition

The process by which the body stores fat in adipose tissue for energy, insulation, and protection of internal organs.

Inflammatory Response

The body's immune response to infection or injury, characterized by redness, swelling, heat, and pain.

Corticotropin-Releasing Factor

A peptide hormone involved in the stress response by stimulating the pituitary gland to release adrenocorticotropic hormone (ACTH).