A Researcher Inserted DNA Fragments from an Organism into Expression

question 58

Multiple Choice

A researcher inserted DNA fragments from an organism into expression vector plasmids and introduced the modified plasmids into bacterial cells. Which of the following methods would be an effective means of identifying which clones contain a specific gene of interest?

• A) Sequence the DNA of the plasmids from each isolate.
• B) Prepare and analyze restriction fragments of the plasmids.
• C) Isolate clones that still have the vector's genetic marker.
• D) Assay for activity of the gene product.
• E) Use reverse transcriptase to prepare cDNA copies.

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Definitions:

Anti-Depressant Medication

Anti-Depressant Medication includes drugs prescribed to treat depressive disorders, working by altering chemicals in the brain to improve mood and cognitive function.

Placebo

A substance that looks and feels like the substance being tested in an experiment, but does not contain the active ingredient.

Moderate Depression

A mental health condition characterized by persistent feelings of sadness, hopelessness, and a lack of interest or pleasure in activities, but to a lesser degree than severe depression.