The Turnover Number for an Enzyme Is Equivalent to the Number

question 9

Multiple Choice

The turnover number for an enzyme is equivalent to the number of substrate molecules processed per second per enzyme molecule. To a test tube containing a 100 mM concentration of its substrate, you have added an enzyme at a final concentration of 10 µM, and have measured the rate of the reaction to be approximately 500 µM/sec. If the Km for the binding of the enzyme to this substrate is about 100 mM, what is the turnover number?

• A) 100
• B) 500
• C) 1000
• D) 5000
• E) 10,000

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