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A Researcher Studying the Human Immunodeficiency Virus (HIV) Has Infected

question 56

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A researcher studying the human immunodeficiency virus (HIV) has infected human T cells with wild-type or Rev-deficient viruses. She extracts cytoplasmic RNA from the cells and separates each isolated RNA mixture by agarose-gel electrophoresis. She then uses northern analysis using HIV-specific probes to identify those bands that contain viral RNA. The results are presented in the following schematic drawing. Which lane (1 or 2) corresponds to cells infected with the Rev?-deficient virus? What is the size of the full-length HIV genome? A researcher studying the human immunodeficiency virus (HIV)  has infected human T cells with wild-type or Rev-deficient viruses. She extracts cytoplasmic RNA from the cells and separates each isolated RNA mixture by agarose-gel electrophoresis. She then uses northern analysis using HIV-specific probes to identify those bands that contain viral RNA. The results are presented in the following schematic drawing. Which lane (1 or 2)  corresponds to cells infected with the Rev?-deficient virus? What is the size of the full-length HIV genome?   A)  Lane 1; about 9000 nucleotides B)  Lane 1; about 4000 nucleotides C)  Lane 1; about 2000 nucleotides D)  Lane 2; about 9000 nucleotides E)  Lane 2; about 2000 nucleotides


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