In Sanger Sequencing, Does Each Labeled DNA Molecule Have a Fluorescently

question 40

Multiple Choice

In Sanger sequencing, does each labeled DNA molecule have a fluorescently labeled dideoxyribonucleotide at its 5′ end or its 3′ end? Do the labeled nucleotides lack the 2′ or 3′ hydroxyl group?

• A) 3′ end; 2′ hydroxyl group only
• B) 3′ end; 3′ hydroxyl group only
• C) 5′ end; 2′ hydroxyl group only
• D) 5′ end; 3′ hydroxyl group only
• E) 3′ end; both hydroxyl groups

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Definitions:

Self-awareness

An understanding of one's personal traits, sentiments, driving factors, and aspirations.

Self-recognition

The cognitive ability to recognize oneself in a mirror or photograph, often considered a milestone in self-awareness and identity development.

Categorical Self

A classification of the self children use to guide their behavior; based on broad ways in which people differ, such as sex, age, and physical characteristics.

Mirror

An object with a reflective surface that forms an image of whatever stands before it, commonly used for personal grooming or decorative purposes.