Consider a Protein Composed of Only 21 Amino Acids Encoded

question 4

Multiple Choice

Consider a protein composed of only 21 amino acids encoded by a short ORF that is located between two known genes in a mammalian genome. Due to its length, the ORF had not been annotated as a gene. Which of the following methods can demonstrate that it is indeed a gene that is actively translated to protein?

• A) RNA-seq
• B) RT-PCR
• C) DNA microarray analysis
• D) ChIP-seq
• E) Ribosome profiling

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