How Is Tandem Affinity Purification Distinct from Other Purification Schemes

question 38

Multiple Choice

How is tandem affinity purification distinct from other purification schemes such as simple affinity or ion-exchange chromatography?

• A) In its use of antibodies
• B) In its use of proteases
• C) In its use of glutathione S-transferase
• D) In its use of viral peptides
• E) In its use of limited washing steps

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Definitions:

Substantial-purpose Rule

A legal doctrine that focuses on the main objective of an action to determine its legality or the applicability of certain laws.

Main-purpose Rule

A legal principle stating that if the main purpose of a promise to pay another's debt is to benefit the promisor, the promise may be enforceable without being in writing.

Executor

An individual appointed in a will to manage the estate of the deceased, ensuring that all affairs are settled and wishes are honored.

Estate

The total property, assets, and liabilities of a person, especially considered in the context of inheritance or estate planning.