You Have Devised Genetic Screens to Identify Gain- and Loss-Of-Function

question 78

Short Answer

You have devised genetic screens to identify gain- and loss-of-function mutations in the Escherichia coli DNA ligase gene. One of the screens (1) seeks mutant cells that are infected more readily with a ligase-deficient strain of T4 phage. The other screen (2) seeks mutant cells that have increased radiation sensitivity at high temperatures. Indicate whether each of the following descriptions better applies to screen 1 (1) or screen 2 (2). Your answer would be a three-digit number composed of digits 1 and 2 only, e.g. 122.
( ) It would identify gain-of-function DNA ligase mutants.
( ) It would identify loss-of-function DNA ligase mutants.
( ) It would identify conditional mutants.

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Definitions:

Federally-Funded

Financial support given by the federal government to support a wide range of projects and programs.

Experimental Realism

The extent to which study participants get so caught up in the procedures that they forget they are in an experiment.

Mundane Realism

The degree to which an experiment or research setting resembles the real-world scenarios it aims to replicate or study.

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Term often used to refer to the practical, everyday life experiences and situations outside of controlled or theoretical environments.