In Sanger Sequencing, Does Each Labeled DNA Molecule Have a Fluorescently

question 40

Multiple Choice

In Sanger sequencing, does each labeled DNA molecule have a fluorescently labeled dideoxyribonucleotide at its 5′ end or its 3′ end? Do the labeled nucleotides lack the 2′ or 3′ hydroxyl group?

• A) 3′ end; 2′ hydroxyl group only
• B) 3′ end; 3′ hydroxyl group only
• C) 5′ end; 2′ hydroxyl group only
• D) 5′ end; 3′ hydroxyl group only
• E) 3′ end; both hydroxyl groups

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Definitions:

Price Taker

A market participant that accepts prevailing market prices as given, without having the power to influence these prices.

Competitive Market

A market structure characterized by many buyers and sellers, free entry and exit, and products that are similar, ensuring no single entity can control prices.

Profits

The financial gain achieved when the revenues generated from business activities exceed the expenses, taxes, and costs incurred in operating the business.

Fixed Costs

Costs that do not vary with the level of production or sales, such as rent, salaries, and insurance premiums.