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A Pool of Five Barcoded Yeast Mutants, Each Deleted for a Different

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A pool of five barcoded yeast mutants, each deleted for a different gene (genes A to E), has been grown under three different conditions (1 to 3). After the growth phase was completed, genomic DNA was isolated from the entire pool and the relative amount of each mutant was determined by quantifying the amount of DNA containing each barcode. The results are presented in the following graph. Which gene (A to E) is essential under condition 1 but not under conditions 2 or 3? A pool of five barcoded yeast mutants, each deleted for a different gene (genes A to E), has been grown under three different conditions (1 to 3). After the growth phase was completed, genomic DNA was isolated from the entire pool and the relative amount of each mutant was determined by quantifying the amount of DNA containing each barcode. The results are presented in the following graph. Which gene (A to E) is essential under condition 1 but not under conditions 2 or 3?


Definitions:

Brainstem

The lower extension of the brain where it connects to the spinal cord, responsible for regulating vital bodily functions such as breathing, heart rate, and blood pressure.

Subarachnoid Space

The space between the arachnoid membrane and the pia mater of the brain and spinal cord, filled with cerebrospinal fluid.

Arachnoid Mater

A delicate membrane that encloses the brain and spinal cord, sitting between the pia mater and the dura mater.

Lateral View

A perspective or representation of an object or structure as seen from the side.

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