Which of the Following Methods Provides the Most Sensitive and Accurate

question 59

Multiple Choice

Which of the following methods provides the most sensitive and accurate way of measuring and comparing mRNA levels of a set of genes in two different tissues. The genes are expressed at relatively low levels in these tissues, and do not necessarily code for protein.

• A) RNA-seq
• B) RT-PCR
• C) DNA microarray analysis
• D) ChIP-seq
• E) Ribosome profiling

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Definitions:

Sample Size

The number of individuals or observations included in a study, which can significantly affect the reliability and validity of the study's outcomes.

Costs

The expenses associated with a project, program, or intervention, including direct, indirect, fixed, and variable costs.

Precision Required

A necessity for accuracy and specificity in data, measurements, or outcomes.

Margin of Error

A statistic expressing the amount of random sampling error in a survey's results, indicating the range of accuracy.