Imagine a Bistable System Composed of Two Repressor Proteins X

question 56

Short Answer

Imagine a bistable system composed of two repressor proteins X and Y, each of which binds to the cis-regulatory sequences of the gene encoding the other protein, i.e. X represses the expression of Y, and Y represses the expression of X. A researcher engineers the genes such that they encode protein X fused to green fluorescent protein (GFP) and protein Y fused to red fluorescent protein (RFP). Cells containing the fusion constructs are then grown and sorted by fluorescence-activated cell sorting (FACS) based on their GFP and RFP signals. The results are presented in the following dot plot. Each cell is represented by a dot whose coordinates correspond to GFP and RFP fluorescence in the cell. According to these results, which protein (X or Y) do you think binds to its target DNA sequence with a higher degree of cooperativity? Write down X or Y as your answer.

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Definitions:

Procedural Unconscionability

A legal doctrine referring to a situation where the manner in which a contract was formed is deemed unjust, due to a significant disparity in power or a lack of fairness.

Contractual Terms

Specific conditions and stipulations outlined in a contract that dictate the obligations and rights of the parties involved.

Legal Jargon

Specialized terminology or technical language used by legal professionals that may be difficult for non-experts to understand.

Institutional Ageism

Discriminatory policies and practices within institutions that favor younger individuals over older ones, often manifesting in employment, healthcare, and media representation.