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Indicate true (T) and false (F) statements below regarding N-linked glycosylation of proteins. Your answer would be a four-letter string composed of letters T and F only, e.g. TTTF.
( ) N-linked glycosylation can be carried out co-translationally, possibly at multiple asparagine residues on the same protein molecule.
( ) N-linked glycosylation is a gradual process, with step-by-step addition and trimming events that commence with the addition of N-acetylglucosamine to an asparagine side chain.
( ) Most proteins synthesized in the rough ER are N-glycosylated, and some of them require this modification for their correct folding.
( ) Once a protein is properly folded in the ER, its attached oligosaccharides are quickly removed by an N-glycanase, although it may be glycosylated again later.
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