You Have Isolated Mitochondria from a Liver Tissue Sample, Suspended

question 29

Multiple Choice

You have isolated mitochondria from a liver tissue sample, suspended them in a hypotonic buffer that causes them to swell and burst their outer membrane, and then added sucrose to a final concentration of about 25%. You then layer the suspension on a density gradient, ultracentrifuge, collect the different fractions, and analyze their protein content. Which of the following proteins would you expect to be highly enriched in the lower density and higher density fractions, respectively?

• A) Cytochrome oxidase; porin
• B) Cytochrome oxidase; ATP synthase
• C) ATP synthase; cytochrome oxidase
• D) Porin; ATP synthase

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Spending Variance

The discrepancy between the budgeted or forecasted expenditure and the actual amount spent.

Client-Visits

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Items used in medical care, including tools, equipment, and consumables, essential for diagnosing, preventing, and treating illnesses.