The Immortalized Non-Malignant Mouse Cell Line NIH-3T3 Was Derived from Normal

question 21

Multiple Choice

The immortalized non-malignant mouse cell line NIH-3T3 was derived from normal mouse fibroblasts in the early 1960s. These cells are able to readily take up exogenous DNA and are prone to transformation by cancer-causing agents, including some retroviruses. DNA extracted from a human bladder carcinoma line is able to transform these cells, as judged by a significant increase in the number of foci (cell clumps) in the cell-culture plates when the DNA is added. The malignant cells contain human DNA, and the DNA can be shown by sequence analysis to contain a single mutant gene that is present in the original bladder carcinoma cell line. The gene codes for a monomeric G protein and was one of the first cancer-critical genes to be identified in this way. The protein encoded by this gene is …

• A) Src
• B) Ras
• C) Myc
• D) p53
• E) Apc

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Definitions:

Aggravating Factors

Conditions or aspects of a case that can increase the severity or culpability of a criminal act, leading to harsher sentences.

Associated Symptoms

Signs or manifestations related to a particular disease or condition, often helping in the diagnosis or assessment of the disease.

Triage

The process of determining the priority of patients' treatments based on the severity of their condition, used in emergency and medical settings.

Phone Screening

An initial interview conducted over the phone to assess a job applicant's qualifications before inviting them for a face-to-face interview.