BrdU (Bromodeoxyuridine) Is a Thymidine Analog That Can Be Naturally

question 34

Short Answer

BrdU (bromodeoxyuridine) is a thymidine analog that can be naturally incorporated into DNA (in place of thymidine) and detected by specific anti-BrdU antibodies. In an experiment to study the regeneration capabilities of Schmidtea mediterranea, you first fed the worms with BrdU-containing food for 30 minutes. After two hours, you fixed and incubated the worms with a fluorescently labeled anti-BrdU antibody, and observed its tissues with a fluorescence microscope. The result was that some nuclei are labeled with fluorescence while others are unlabeled. Would you expect to observe neoblasts mostly among cells that have (Y) or have not (N) been labeled with fluorescence? Are the neoblasts expected to be scattered throughout the body (S) or localized to a certain region (L)? If you fixed the worms after a week, instead of two hours, would you expect the number of nuclei with detectable fluorescence to increase (I), remain unchanged (U), or decrease (D) compared to these results? Write down your answer as a three-letter string, e.g. YSD.

---

Definitions:

Physical Environment

The physical environment encompasses all the external, tangible surroundings in which an organism lives, including both natural and built environments.

Personal Space

A zone of space surrounding a person that is felt to “belong” to that person.

Communication Process

The communication process is the sequence of steps through which information is conveyed from a sender to a receiver, encompassing message creation, transmission, reception, and interpretation.

Sender

The entity in communication who is responsible for creating and transmitting a message to a receiver.