The Melting Profile for a DNA Is Found to Differ

question 35

Multiple Choice

The melting profile for a DNA is found to differ in two different salt solutions. In 0.001 M NaCl, T_m = 55 ° C, and in 0.01 M NaCl, T_m = 65 ° C. The fundamental reason for this change is

• A) higher salt concentration increases the electrostatic repulsions between phosphates
• B) the base stacking is more effective in high salt
• C) hydrogen bonding between complementary bases in enhanced at high salt
• D) higher salt concentration decreases the electrostatic repulsions between phosphates

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