Exhibit 13A This Is a Map of the PBR322 Plasmid

question 37

Multiple Choice

Exhibit 13A This is a map of the pBR322 plasmid, numbers on the map refer to the base where the site is located. Refer to Exhibit 13A. If a recombinant plasmid was obtained inserting DNA into the Bam HI site, screening for the recombinant plasmid can be done by the following technique.

• A) Plate on nutrient agar plates that contain ampicillin.
• B) Plate on nutrient agar plates that contain tetracycline.
• C) Plate on nutrient agar plates that contain both ampicillin and tetracycline.
• D) Plate on nutrient agar plates that contain ampicillin, followed by replica plating on tetracycline.
• E) Plate on nutrient agar plates that contain tetracycline, followed by replica plating on ampicillin.

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