Exhibit 13A This Is a Map of the PBR322 Plasmid

question 80

Multiple Choice

Exhibit 13A This is a map of the pBR322 plasmid, numbers on the map refer to the base where the site is located. Refer to Exhibit 13A. If a recombinant plasmid were obtained inserting DNA into the Afl III site, the recombinant plasmid would lack which of the following properties?

• A) It can replicate autonomously.
• B) It will be resistant to the antibiotic tetracycline.
• C) It will be resistant to the antibiotic ampicillin.
• D) It will possess at lest two promoter sites.
• E) All of these are true for the recombined plasmid.

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Definitions:

Terrible Sufferings

Experiences of extreme pain, distress, or hardship.

First Cause

A philosophical concept suggesting that everything has a cause, and there must be an initial uncaused cause that started the chain of events in the universe.

Always Existed

A concept referring to something that has had no beginning, and has been in existence for an infinite amount of time.

Baruch Spinoza

A Dutch philosopher of Portuguese Sephardi origin, known for his work in ethics, epistemology, and metaphysics, emphasizing monism – the idea that there is only one substance in the universe.