Exhibit 13D the Plasmid PSU922 Is a Circular DNA Containing

question 4

Multiple Choice

Exhibit 13D The plasmid pSU922 is a circular DNA containing 25000 base pairs. The β -gal gene codes for the enzyme β -galactosidase, the product of which will turn bacterial colonies blue when grown in the presence of X-gal; the Amp^R gene confers ampicillin resistance. Refer to Exhibit 13D. Neglecting any discussion of whether it's a good or bad choice, I attempt to insert a gene fragment into the Hind III site and transform bacteria with the plasmid. How can I tell which transformants have the insert?

• A) The bacteria will not be able to grow in the presence of ampicillin, and they will be blue.
• B) The bacteria will not be able to grow in the presence of ampicillin, and they will be white.
• C) The bacteria will be able to grow in the presence of ampicillin, and they will be blue.
• D) The bacteria will be able to grow in the presence of ampicillin, and they will be white.

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Family Functions

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