Sometimes DNA Probes Are Used to Prove That a Gene

question 52

Multiple Choice

Sometimes DNA probes are used to prove that a gene has been incorporated into a eukaryotic genome. The following are all steps used in such a procedure, except :

• A) Digestion of the cellular DNA to break it into manageable sizes.
• B) Separation of the fragments by gel electrophoresis.
• C) Hybridization of a nucleic acid strand complementary to the gene of interest.
• D) Elution of the hybridized DNA from the gel for analysis.
• E) All of these steps are necessary in probing for the presence of a gene.

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Definitions:

Blind

Blindness is the condition of lacking visual perception due to physiological or neurological factors.

Faster

Operating or moving at high speed or more quickly than is usual, often used to compare the speed or efficiency of processes, objects, or people.

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Exposure to artificial light that changes activity patterns and so disrupts circadian rhythms.

Circadian Rhythms

Biological processes that display an endogenous, entrainable oscillation of about 24 hours, influencing sleep-wake cycles, hormone release, and other bodily functions.