Lederberg and Tatum Used the Bacterial Strains Bio- Met- Leu

question 42

Multiple Choice

Lederberg and Tatum used the bacterial strains bio- met- leu+ thr+ thi+ and bio+ met+ leu- thr- thi- in their experiments to determine if genetic recombination occurs in bacteria. Would it have been better to use two strains with the genotypes met- leu+ and met+ leu- instead? ​

• A)Yes, because it is easier to study 2 genes than 5 genes.
• B)Yes, because the met- leu+ and met+ leu- strains only have one mutation, which makes it easier for genetic recombination to occur. ​
• C)No, because the met- leu+ and met+ leu- strains only have one mutation. A single random mutation could restore methionine or leucine synthesis in the strains.
• D)No, because strains with only one nutrient-deficient gene can still grow on minimal medium.
• E)No, because cells have two genes that encode different versions of leucine, so the met+ leu- strain would still be able to grow on minimal medium.

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