A Plasmid Cloning Vector and DNA Fragments Containing the XYZ

question 49

Multiple Choice

A plasmid cloning vector and DNA fragments containing the XYZ gene, each digested with the same restriction enzyme, are mixed with DNA ligase. Which product(s) will result? ​

• A)recombinant plasmids only
• B)nonrecombinant plasmids only
• C)joined-together copies of the DNA fragment containing the XYZ gene only
• D)a mixture of recombinant plasmids, nonrecombinant plasmids, and joined-together copies of the DNA fragment containing the XYZ gene
• E)no reaction will occur because DNA polymerase rather than DNA ligase is needed to insert the DNA fragments into the plasmid cloning vector

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