How Do Scientists Determine the Annealing Temperature Used in PCR

question 9

Multiple Choice

How do scientists determine the annealing temperature used in PCR reactions? ​

• A)It is always 55ᵒC because the DNA polymerase works best at this temperature.
• B)It is always 55ᵒC because primers anneal best at lower temperatures.
• C)It is always 60ᵒC because any other temperature would destabilize the DNA.
• D)It can vary from 55-60ᵒC depending on the primers being used.
• E)It can vary from 55-60ᵒC depending on the type of DNA polymerase being used.

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