Identify the Correct Steps for Cloning a Gene of Interest

question 82

Multiple Choice

Identify the correct steps for cloning a gene of interest, then arrange them in the correct order.
1.  Transform the bacteria.
2.  Incubate the DNA fragments and cut cloning vector with DNA ligase.
3.  Use restriction enzymes to cut the gene of interest and the cloning vector.
4.  Spread bacteria on medium containing lactose and ampicillin.
5.  Spread bacteria on medium containing X-gal and ampicillin.

• A)4, 3, 1, 5
• B)3, 2, 5, 1
• C)3, 2, 1, 5
• D)2, 3, 1, 5
• E)3, 2, 1, 4

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Definitions:

Industry Average

A statistical measure that represents the average performance or output of companies within a particular sector or industry.

Adjusting Entries

Journal entries made at the end of an accounting period to update account balances before the preparation of financial statements, ensuring records adhere to the accrual basis of accounting.

Balance Sheet

A declaration that provides a snapshot of a company's assets, financial obligations, and shareholder equity at a designated time.

Total Assets

The sum of all assets owned by a business, representing its total resources.