What Enables a PCR-Amplified Gene to Be Inserted into a Cloning

question 69

Multiple Choice

What enables a PCR-amplified gene to be inserted into a cloning vector?

• A) The PCR reaction adds sticky ends onto the amplified DNA, allowing ligation to a cloning vector.
• B) Restriction enzyme sites are added to the 5' ends of both primers; therefore, digestion of the PCR-amplified gene allows ligation to a cloning vector cut with the same restriction enzyme.
• C) The PCR-amplified gene is sequenced to locate restriction sites in order to determine which restriction enzymes to use for cloning.
• D) The PCR-amplified gene is cut with one restriction enzyme, then ligated to a cloning vector cut by a different restriction enzyme.
• E) The cloning vector is added to the PCR reaction, and once the gene is amplified, it immediately ligates to the cloning vector.

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