Identify the Correct Steps for Cloning a Gene of Interest

question 82

Multiple Choice

Identify the correct steps for cloning a gene of interest, then arrange them in the correct order.
1.  Transform the bacteria.
2.  Incubate the DNA fragments and cut cloning vector with DNA ligase.
3.  Use restriction enzymes to cut the gene of interest and the cloning vector.
4.  Spread bacteria on medium containing lactose and ampicillin.
5.  Spread bacteria on medium containing X-gal and ampicillin.

• A)4, 3, 1, 5
• B)3, 2, 5, 1
• C)3, 2, 1, 5
• D)2, 3, 1, 5
• E)3, 2, 1, 4

Identify the elements needed to calculate standard deviation and its relevance in data interpretation.

Differentiate between types of distributions and their characteristics.

Grasp the relationship between the mean, median, and the presence of outliers in a dataset.

Comprehend the significance of p-values in indicating the probability of results occurring by chance.

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