Which Methodology Was Used by Researchers to Identify the Nuclear

question 66

Multiple Choice

Which methodology was used by researchers to identify the nuclear localization sequence?

• A) infection of cells in culture with viruses containing sequential deletions of amino acids in a protein known to localize to the nucleus, followed by microscopic determination of cellular localization
• B) treatment of cells in culture with pharmacological agents that inhibit the nuclear pore complex, followed by microscopic determination of cellular localization
• C) transfection of cells in culture with mutant human proteins containing sequences believed to be required for nuclear localization followed by microscopic determination of cellular localization
• D) fluorescently labeling cells with known nuclear proteins and tracking changes in localization in the presence or absence of nuclear pore complex proteins
• E) treatment of cells in culture with pharmacological agents that binds to the nuclear localization sequence and prevents nuclear transport, followed by microscopic determination of cellular localization

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Definitions:

Bronchi

Major passages of the respiratory system that diverge from the windpipe (trachea) and direct air into the lungs.

Cilia

Microscopic, hair-like structures on the surface of cells that move in a waving motion to transport substances across the cell surface.

Epiglottis

A flap of tissue at the root of the tongue that is depressed during swallowing to cover the opening of the windpipe and prevent food from entering the lungs.

Larynx

The voice box located in the throat, responsible for producing vocal sounds, protecting the trachea during swallowing, and regulating airflow to the lungs.