Which Methodology Was Used by Researchers to Identify the Nuclear

question 66

Multiple Choice

Which methodology was used by researchers to identify the nuclear localization sequence?

• A) infection of cells in culture with viruses containing sequential deletions of amino acids in a protein known to localize to the nucleus, followed by microscopic determination of cellular localization
• B) treatment of cells in culture with pharmacological agents that inhibit the nuclear pore complex, followed by microscopic determination of cellular localization
• C) transfection of cells in culture with mutant human proteins containing sequences believed to be required for nuclear localization followed by microscopic determination of cellular localization
• D) fluorescently labeling cells with known nuclear proteins and tracking changes in localization in the presence or absence of nuclear pore complex proteins
• E) treatment of cells in culture with pharmacological agents that binds to the nuclear localization sequence and prevents nuclear transport, followed by microscopic determination of cellular localization

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