During DNA Cloning, Why Is the DNA of Interest Inserted

question 42

Multiple Choice

During DNA cloning, why is the DNA of interest inserted into the middle of the lacZ⁺coding sequence in the plasmid vector?

• A) It makes the bacteria resistant to the antibiotic.
• B) It allows researchers to distinguish bacteria that contain the plasmid from those that do not.
• C) It aids scientists in developing new antibiotic treatments.
• D) It allows researchers to distinguish bacteria that contain the recombinant plasmid from those that contain just the vector.
• E) It makes the bacteria produce more clones.

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