A Plasmid Cloning Vector and DNA Fragments Containing the XYZ

question 78

Multiple Choice

A plasmid cloning vector and DNA fragments containing the XYZ gene, each digested with the same restriction enzyme, are mixed with DNA ligase. Which product(s) will result?

• A) Recombinant plasmids only
• B) Nonrecombinant plasmids only
• C) Joined-together copies of the DNA fragment containing the XYZ gene only
• D) A mixture of recombinant plasmids, nonrecombinant plasmids, and joined-together copies of the DNA fragment containing the XYZ gene
• E) No reaction will occur because DNA polymerase, rather than DNA ligase, is needed to insert the DNA fragments into the plasmid cloning vector

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