How Do Scientists Determine the Annealing Temperature Used in PCR

question 76

Multiple Choice

How do scientists determine the annealing temperature used in PCR reactions?

• A) It is always 55^ᵒC because the DNA polymerase works best at this temperature.
• B) It is always 55^ᵒC because primers anneal best at lower temperatures.
• C) It is always 65^ᵒC because any other temperature would destabilize the DNA.
• D) It can vary from 55-65^ᵒC depending on the primers being used.
• E) It can vary from 55-65^ᵒC depending on the type of DNA polymerase being used.

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