What Enables a PCR-Amplified Gene to Be Inserted into a Cloning

question 69

Multiple Choice

What enables a PCR-amplified gene to be inserted into a cloning vector?

• A) The PCR reaction adds sticky ends onto the amplified DNA, allowing ligation to a cloning vector.
• B) Restriction enzyme sites are added to the 5' ends of both primers; therefore, digestion of the PCR-amplified gene allows ligation to a cloning vector cut with the same restriction enzyme.
• C) The PCR-amplified gene is sequenced to locate restriction sites in order to determine which restriction enzymes to use for cloning.
• D) The PCR-amplified gene is cut with one restriction enzyme, then ligated to a cloning vector cut by a different restriction enzyme.
• E) The cloning vector is added to the PCR reaction, and once the gene is amplified, it immediately ligates to the cloning vector.

---

Definitions:

Productivity

The efficiency at which goods and services are produced, often measured by output per unit of input over a specific period.

Destabilizing Forces

Factors or elements that threaten the stability or balance of systems, organizations, or societal conditions.

Food Desert

Areas with limited access to affordable and nutritious food, particularly impacting low-income neighborhoods and communities.

Obesity

A medical condition characterized by excess body fat that presents a risk to health.