40 Identify the Correct Steps for Cloning a Gene of Interest

question 30

Multiple Choice

40. Identify the correct steps for cloning a gene of interest, then arrange them in the correct order. One of the steps below is left out of the answer. 1. Transform the bacteria.
2)  Incubate the DNA fragments and cut cloning vector with DNA ligase.
3)  Use restriction enzymes to cut the gene of interest and the cloning vector.
4)  Spread bacteria on medium containing lactose and ampicillin.
5)  Spread bacteria on medium containing X-gal and ampicillin.

• A) 4, 3, 1, 5
• B) 3, 2, 5, 1
• C) 3, 2, 1, 5
• D) 2, 3, 1, 5
• E) 3, 2, 1, 4

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Definitions:

Required Rate Of Return

The minimum expected yield by investors to justify the risk of an investment.

Investment Opportunity

An investment opportunity refers to potentially lucrative financial ventures or assets that individuals or entities can invest in, aiming for future monetary returns.

Turnover

A metric indicating the rate at which a company's inventory is sold and replaced over a given period.

Combined Turnover

A financial ratio that combines and assesses two or more turnover metrics, such as inventory turnover and receivables turnover, to evaluate the efficiency of a business's operations.