In the Microarray Shown in the Textbook (P

question 25

Multiple Choice

In the microarray shown in the textbook (p. 414) , the cDNAs were labeled with red and green fluorescent tags. Why do some spots on the microarray emit yellow light?

• A) Red and green are two of the primary colors, and if both cDNAs hybridize to the spot, the combination of the two will produce yellow light.
• B) The yellow light comes from the laser, and a yellow spot indicates that neither cDNA hybridized to that spot on the microarray.
• C) The over-expression of one cDNA relative to the other will skew the color pattern of the spot on the microarray, resulting in the yellow color.
• D) The color choice was an arbitrary decision by the artist and doesn't reflect how the process actually works.
• E) Yellow spots are those that contain no DNA probes on the chip.

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Definitions:

One-sample T-test

A statistical test used to determine if the mean of a single sample differs significantly from a known or hypothesized population mean.

Parametric Counterpart

Statistical methods or models that assume the data comes from a population that can be characterized by a set of parameters.

Friedman Test

A non-parametric test used to detect differences in treatments across multiple test attempts, especially for matched or paired subjects.

Kruskal-Wallis Test

A statistical test that determines if three or more independent samples come from populations with the same distribution, using ranking methods.