A Chemist Was Performing a Spectroscopy Experiment on a Fluorescent

question 29

Multiple Choice

A chemist was performing a spectroscopy experiment on a fluorescent dye to examine the influence of concentration on absorbance. To accomplish this, the chemist created a stock solution of a certain concentration of dye using 10 mL of water (H₂O) . Then, a serial dilution was performed by taking 1 mL of the stock solution and diluting it with 9 mL of water for a total solution volume of 10 mL. 1 mL was taken from Solution 2 and diluted with 9 mL water to create Solution 3. The serial dilution was performed through Solution 5, as shown in the diagram below. If the dye concentration in Solution 5 was 0.5 g/mL, what was the concentration of the dye in Solution 2?

• A)50 g/mL
• B)500 g/mL
• C)5 gmL
• D)5,000 g/mL

---

Definitions:

Halogens

A group of highly reactive elements located in Group 17 of the periodic table, which include fluorine, chlorine, bromine, iodine, and astatine.

Atomic Symbol

A one- or two-letter notation used to represent an element, based on its Latin name.

Copper

A red-orange metallic element that is ductile and conductive, used extensively in electrical wiring and plumbing.

Atomic Symbol

A one or two-letter notation representing a chemical element (e.g., H for hydrogen, O for oxygen) based on its name, often derived from Latin.