Researchers Lyse Human Cells and Isolate a Specific Messenger RNA

question 33

Multiple Choice

Researchers lyse human cells and isolate a specific messenger RNA template using gel electrophoresis.  Reverse transcription polymerase chain reaction is then used to synthesize complementary DNA (cDNA) from the RNA template.  Next, the cDNA is modified into an expression vector containing an optimized bacterial promoter, ribosomal binding site, and terminator sequence.  After insertion of the vector into appropriate bacterial hosts, the transformed bacteria are cultured in a bioreactor and produce large quantities of a protein containing a domain that binds to a specific DNA sequence.  This protein is most likely the receptor for which of the following hormones?

• A)Glucagon
• B)Growth hormone
• C)Insulin
• D)Insulin-like growth factor
• E)Parathyroid hormone
• F)Progesterone

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Definitions:

Diencephalon

The portion of the brain that contains structures such as the thalamus and hypothalamus, involved in sensory and autonomic functions.

Subthalamic Nuclei

A small group of cells located in the brain that are part of the basal ganglia system, involved in regulating movements.

Pineal Body

A small gland in the brain involved in the production of the hormone melatonin, regulating sleep patterns.

Third Ventricle

A narrow, midline cavity in the brain, part of the ventricular system where cerebrospinal fluid is produced and circulates, located between the two halves of the thalamus.