As Part of a Research Study Investigating Enzymatic Activity in Both

question 21

Multiple Choice

As part of a research study investigating enzymatic activity in both normal and diseased liver tissue, hepatocytes are isolated from biopsy samples obtained from patients undergoing routine care at a local tertiary referral center.  The cells are homogenized and centrifuged to remove membrane components and organelles.  Following subsequent rounds of centrifugation, the remaining supernatant contains only cytosol and cytosolic proteins.  Activity of which of the following enzymes will most likely be detectable in the supernatant of healthy liver cells?

• A)3-Hydroxy-3-methylglutaryl-CoA lyase
• B)Ornithine transcarbamylase
• C)Pyruvate carboxylase
• D)Succinate dehydrogenase
• E)Transketolase

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Definitions:

Regiochemistry

The branch of chemistry that deals with the direction or orientation of chemical bond formation or breaking during a chemical reaction.

Kinetic Conditions

Conditions that affect the rate of a chemical reaction, focusing on temperature and concentration of reactants.

Organic Reaction

A chemical reaction involving organic compounds, contributing to the formation or breaking of carbon-based chemical bonds.

Cycloaddition

A chemical reaction in which two or more unsaturated molecules (or parts of the same molecule) combine to form a cyclic product.