A Microbiologist Performs a Genetic Experiment in Which Cultures of Escherichia

question 128

Multiple Choice

A microbiologist performs a genetic experiment in which cultures of Escherichia coli are treated with a chemical that induces a high frequency of mutations.  Individual bacterial colonies are isolated to identify a mutant strain that lacks a specific enzyme involved in DNA replication.  This specific enzyme is responsible for removing short fragments of RNA that are base paired to the DNA template.  Which of the following enzymes is most likely deficient in this strain of E coli?

• A)DNA polymerase I
• B)DNA polymerase III
• C)Gyrase
• D)Helicase
• E)Ligase
• F)Primase

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Definitions:

Complete Participant

A researcher fully participates in the social setting under study without revealing their research intentions.

Covert Observation

Observation in a research context where the subject is unaware they are being observed, to ensure natural behavior is recorded.

Overt Observation

A research method where the subjects are aware that they are being observed, which can impact their behavior but also adheres to ethical transparency.

Participant Observation

A qualitative research method involving the researcher immersing themselves in a group or community to observe behaviors and interactions firsthand.