In an Experiment, Cultured Fibroblasts Are Mechanically Lysed, and the Membrane

question 80

Multiple Choice

In an experiment, cultured fibroblasts are mechanically lysed, and the membrane lipids and cellular proteins are chemically removed to isolate nucleic acids.  The cellular extract containing the purified nucleic acids is incubated along with short sequences of repeated deoxythymidine residues fixed to latex beads.  The solution is washed several times to remove unbound molecules.  Which of the following types of nucleic acid is most likely to bind the strongest to the latex beads in this experiment?

• A)Aminoacyl-tRNA
• B)Mature mRNA
• C)Promoter regions of DNA
• D)Ribosomal RNA
• E)Splice sites of pre-mRNA
• F)Telomere regions of chromosomes

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