Bacteria Are Grown in ¹⁵N (Heavy)medium and Then Transferred to ¹⁴N

question 29

Multiple Choice

Bacteria are grown in ¹⁵N (heavy) medium and then transferred to ¹⁴N (light) medium and are allowed to replicate for 2 generations.The DNA is subsequently isolated and centrifuged in a CsCl₂ gradient to yield what type of gradient band(s) ?

• A)one heavy band
• B)one light band
• C)one half-heavy (intermediate to heavy and light)band
• D)one heavy and one half-heavy band
• E)one light and one half-heavy band

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