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In Recombinant DNA Technique,what Enzyme Is Needed to Seal the Sticky

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In recombinant DNA technique,what enzyme is needed to seal the sticky ends of genes into plasmids or chromosomes?

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Definitions:

Single Cell

Pertaining to an organism or entity that consists of only one cell, often referring to unicellular organisms.

Foliose

A type of lichen that has a leaf-like appearance, typically loosely attached to its substrate by the middle, allowing some movement.

Crustose

Crustose refers to a type of lichen that has a crust-like growth form, adhering closely to the substrate such as rocks or tree bark.

Fruticose

Pertaining to lichens that have a shrubby, branched structure.

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