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The Table Represents a Specific Substrate Concentrations for an Enzyme

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Essay

The table represents a specific substrate concentrations for an enzyme that displays classical Michaelis-Menten kinetics. Two sets of inhibitor data are also included. Determine the Km and Vmax for the uninhibited enzyme.
 Without Vo(mM/s)[S](mM) Inhibitor  With Inhibitor A  With Inhibitor B 1.32.501.170.622.64.002.101.426.56.304.002.6513.07.605.703.1226.09.007.203.58\begin{array}{l} \quad\quad\quad\quad\quad\text { Without } \quad\quad\quad\quad\quad\quad\quad\quad {\mathrm{V}_{\mathrm{o}}(\mathrm{mM} / \mathrm{s})} \\\begin{array}{llcc} {[\mathrm{S}](\mathrm{mM})} & \text { Inhibitor } & \text { With Inhibitor A } & \text { With Inhibitor B } \\1.3 & 2.50 & 1.17 & 0.62 \\2.6 & 4.00 & 2.10 & 1.42 \\6.5 & 6.30 & 4.00 & 2.65 \\13.0 & 7.60 & 5.70 & 3.12 \\26.0 & 9.00 & 7.20 & 3.58\end{array}\end{array}


Definitions:

Keystone Species

A species that plays a critical role in maintaining the structure of an ecological community, affecting many other organisms in the ecosystem.

Local Environment

The immediate physical and biological surroundings of an organism, influencing its growth and development.

Change

Change refers to the process of becoming different in some particular way, without permanently losing one's or its former characteristics or essence.

Keystone Species

An essential species in an ecosystem that plays a key role in maintaining the balance, wherein its elimination would cause dramatic shifts in the ecosystem's dynamics.

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