In an Experiment to Investigate the Inhibition of the Enzyme-Glucosidase

question 12

Multiple Choice

In an experiment to investigate the inhibition of the enzyme-glucosidase the following data for the rates of reaction with glucopyranoside for various substrate concentrations was obtained. By constructing a Leaver-Burk plot, determine the value of the Michaelis constant.
$\begin{array}{lllll} [S]/\left(10^{-8} \mathrm{~mol} \mathrm{dm}^{-3}\right) & 1.00 & 2.00 & 3.00 & 4.00 \\V/\left(10^{-3} \mathrm{~mol} \mathrm{dm}^{-3} \mathrm{~s}^{-1}\right) & 16.7 & 33.3 & 41.1 & 49.8\end{array}$

• A) 8.46 ? 10^-6 mol dm^-3
• B) 8.46 mol dm^-3
• C) 8.46 ? 10³ mol dm^-3
• D) 8.46 ? 10⁶ mol dm^-3

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