What Is One Major Obstacle That Using the Transcriptome (Single-Cell

question 23

Multiple Choice

What is one major obstacle that using the transcriptome (single-cell transcriptomics) would present in classifying neurons?

• A) The transcriptome of any cell is dynamic-it changes constantly, depending on the demands of the cell and any point in time, as well as on the type of mRNA being classified (e.g., spliced? processed?)
• B) Technology for this practice is still not advanced enough.
• C) The RNA-Seq database applies only to neurons of the same overall function, such as all cortical neurons or all hippocampal neurons or all cerebellar neurons.
• D) mRNA is too unstable and difficult to work with successfully.
• E) This technology is fraught with logistical problems, such as corroborating the same results from different labs.

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